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. 2021 Feb 1;118(6):e2019409118. doi: 10.1073/pnas.2019409118

Fig. 4.

Fig. 4.

CBD functions as a neuromodulator of pBLA-vCA1 innervation in CUMS-induced depressive-like behaviors. (A) Experimental design. (B) Representative images of dendrites, traced neuronal processes, and spines in vCA1 in each group. (C) Quantification of dendritic intersection (two-way ANOVA with Sidak’s post hoc test, **P < 0.01, ***P < 0.001, NS+Veh+Veh vs. CUMS+Veh+Veh group; n = 12 neurons from n = 4 mice per group; Sholl analysis). (D) Quantification of total and mushroom spines in vCA1 (one-way ANOVA with Tukey’s post hoc test, *P < 0.05, **P < 0.01, #P < 0.05, &P < 0.05; n = 36 dendritic segments from n = 4 mice per group). (E) Representative images of Western blots. NVV, NS+Veh+Veh; CVV, CUMS+Veh+Veh; CCV, CUMS+CBD+Veh; CVA, CUMS+Veh+AM251; CCA, CUMS+CBD+AM251. (F) Quantification of synaptosomal (“S”) GluA1, GluA2, and PSD95 and total (“T”) pS831GluA1, pS845GluA1, pT286CaMKIIα, and CaMKIIα in vCA1 (one-way ANOVA with Tukey’s post hoc test, *P < 0.05, **P < 0.01, CUMS+Veh+Veh vs. NS+Veh+Veh group; ##P < 0.01, ###P < 0.001, CUMS+CBD+Veh vs. CUMS+Veh+Veh group; &P < 0.05, CUMS+CBD+AM251 vs. CUMS+CBD+Veh group). (G) Representative images and quantification of DIO-mCherry (red) and c-Fos (green) double-positive cells in vCA1. White arrowheads indicate colabeled cells (one-way ANOVA with Tukey’s post hoc test, *P < 0.05, **P < 0.01, #P < 0.05). (H) Immobility (Left) and latency time (Right) in FST after CBD treatment (one-way ANOVA with Tukey’s post hoc test, *P < 0.05, **P < 0.01, #P < 0.05). Data are presented as the mean ± SEM.