Fig. 3.

Suppression of the NF-κB signaling pathway reduces the proliferation and viability of PCa cells treated with EZH2 inhibitors. a LNCaP cells were transfected with pGL3-NFkB-Luc and pSV40-renilla plasmids for 12 h and then treated with either 10 μM EPZ6438 and/or 10 μM BAY11-7082 for additional 24 h. The luciferase activity was then measured. **P < 0.01. b The cell growth curve of LNCaP cells treated with or without either 5 μM EPZ6438 and/or 5 μM BAY11-7082 for the indicated days. ***P < 0.001. c LNCaP cells were treated with or without either 10 μM EPZ6438 and/or 10 μM BAY11-7082 for 24 h. CCK-8 was then added and the absorbance at A450 was determined by a microplate reader. *** P < 0.001. d PCa cell lines PC3, 22RV1 and Du-145 were treated with or without either 10 μM GSK126/EPZ6438 and/or 10 μM BAY11-7082 for 24 h. CCK-8 was then added and the absorbance at A450 was determined by a microplate reader. ***P < 0.001. e LNCaP cells were transfected with indicated siRNAs for 24 h and then treated with or without 10 μM BAY11-7082 for additional 24 h. The luciferase activity was then measured. ***P < 0.001