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. 2020 May 7;106(4):1000–1007. doi: 10.3324/haematol.2019.235150

Figure 1.

Figure 1.

Generation of the Asxl1G643Wmutant mouse line. (A) Schematic representation of the three last exons of the Asxl1 gene. The red star represents the G643W mutation. The “g” inserted in the mutated allele is indicated in red. The protein sequence is in capitals and the asterisk represents the stop codon generated as a result of the frameshift. The blue arrows represent primers used for genotyping (two forward primers have been used to selectively anneal the wild-type [WT] and mutated sequence, respectively). (B) Schematic representation of WT and G643W mutant ASX1 proteins. The major plant homeodomain (PHD) and additional sex combs homology domain (ASXH) are indicated. Amino acids are numbered. The light blue arrows represent the primers used for quantitative RT-PCR. (C) The relative expression of Asxl1 cDNA in Asxl1+/+, Asxl1G643W/+ and Asxl1G643W/G643W BM cells, determined by quantitative RT-PCR (n=3 mice in each experimental group).