Table 1.

Clinical measurements and collection of biomaterials

Measurements	Description
Anthropometry	Height: Stadiometer without shoes Weight: Scale (Soehnle, Nassau, Germany) without shoes or heavy clothing Waist and hip circumference: Measuring tape (SECA, Birmingham, UK)
Ankle–brachial-index	Doppler (Sonotrax Basic A, Edan, San Diego, CA, U.S.)
Blood pressure	IntelliVue MP5SC (Philips, Amsterdam, Netherlands)
Electrocardiogram	Cardiosoft V6.73 (GE Healthcare, Buckinghamshire, UK)
Spirometry	EasyOne® ultrasonic spirometer (ndd Medical, Zürich, Switzerland)
eNO	NIOX VERO® (Aerocrine AB, Solna, Sweden)
CT scans	CT angiography, CACS, CT chest, CT upper abdomen. 320-detector MSCT (Canon Aquilion ONE, PRISM, Canon Medical Systems, Otawara, Japan)
Transthoracic echocardiography	Vivid 9 Ultrasound System (GE Healthcare, Horten Norway) M-mode, colour tissue doppler, conventional spectral doppler, 2D speckle tracking, 3-dimensional echocardiography
Biobank	PBMC, plasma, serum

CACS coronary artery calcium score, CT computed tomography, MSCT multi-slice computed tomography, PBMC peripheral blood mononuclear cell