Table 1.

Interaction kinetics of pHis Fabs with pTza and pHis peptides

Fab type	ACLYana-1/3-pTza peptide (AGAG-1/3-pTza-AGAG) - Cognate type	ACLYana-1/3-pHis peptide (AGAG-1/3-pHis-AGAG)
SC1-1
KD, M	2.48 × 10−8 ± 1.14 × 10−10	9.51 × 10−8 ± 5.56 × 10−10
ka, 1/Ms	8.13 × 104 ± 3.47 × 102	9.73 × 104 ± 5.51 × 102
kd, 1/s	1.99 × 10−3 ± 3.19 × 10−6	9.25 × 10−3 ± 1.33 × 10−5
SC50-3
KD, M	1.6 × 10−8 ± 6.8 × 10−11	2.83 × 10−8 ± 1.75 × 10−10
ka, 1/Ms	8.51 × 104 ± 3.03 × 102	1.75 × 105 ± 1.01 × 103
kd, 1/s	1.36 × 10−3 ± 3.19 × 10−6	4.94 × 10−3 ± 1.06 × 10−5
SC39-4
KD, M	6.7 × 10−8 ± 4.47 × 10−10	7.99 × 10−7 ± 3.33 × 10−8
ka, 1/Ms	1.64 × 105 ± 1.06 × 103	1.47 × 105 ± 5.78 × 103
kd, 1/s	1.1 × 10−2 ± 1.6 × 10−5	1.17 × 10−1 ± 1.6 × 10−3
SC44-8
KD, M	2.35 × 10−10 ± 4.87 × 10−12	1.82 × 10−8 ± 7.15 × 10−11
ka, 1/Ms	1.56 × 105 ± 4.57 × 102	9.36 × 104 ± 3.19 × 102
kd, 1/s	3.66 × 10−5 ± 7.5 × 10−7	1.7 × 10−3 ± 3.32 × 10−6
SC56-2
KD, M	9.66 × 10−8 ± 1.11 × 10−9	2.42 × 10−6 ± 7.36 × 10−8
ka, 1/Ms	9.73 × 104 ± 1.11 × 103	7.56 × 104 ± 2.17 × 103
kd, 1/s	9.39 × 10−3 ± 1.78 × 10−5	1.83 × 10−1 ± 1.81 × 10−3