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Synthesis of VLC-PUFA 32:6 n-3. (A) Synthetic approach to 32:6 n-3. (B) GC-MS chromatogram of synthetic 32:6 n-3 in comparison with bovine retinal lipids. (C) High-resolution mass spectral confirmation of the molecular weight of the synthetic 32:6 n-3. Calculated molecular weight for C32H52O2Na [M+Na]+ (m/z) 491.3865; found 491.3857.
