Fig. 5.
KRAS PBD mutant anchors respond to changing PM curvature in distinct manners. BHK cells ectopically coexpressing GFP-KRASG12V, or a GFP-KRASG12V PBD mutant anchor, together with RFP-pinkBAR, an empty vector pC1, or RFP-BARamph2 to induce low curvature, normal, or high curvature, respectively, were grown to ∼80% confluency. PM sheets were prepared on EM grids and immunogold labeling using anti-GFP coupled to 4.5-nm gold. Univariate K-functions were used to analyze the spatial distributions of each anchor. Mean Lmax values (± SEM) for each KRAS anchor (A) calculated from 12 ≤ n ≤ 16 PM sheets selected from different cells. Significant differences between Lmax values were assessed using bootstrap tests (*P < 0.05). (B) Mean number of gold particles per μm2 (± SEM) calculated for the same set of PM sheets. Significant differences between values were assessed using one-way ANOVA (*P < 0.05).