Fig. 6.

Trisomic primary fibroblasts up-regulate the de novo synthesis of sphingolipids. (A) The expression levels of subunits SPTLC1 and SPTLC2 of the serine palmitoyltransferase enzyme in fibroblasts in this study and in the Sullivan et al. (20) and Letourneau et al. (16) studies. (B) The expression levels of subunits SPTLC1 and SPTLC2 in T cells and monocytes (20). (C) The protein levels of SPTLC1 and SPTLC2 in primary fibroblasts. (D) The Western blots of SPTLC1 and SPTLC2 in primary fibroblasts. GADPH was used as a loading control.