Table 1.

Composition and property of biomolecular condensates observed in this study

Condensates	Formation	Example figure	Tested condensate components	Properties
Stress granules	Induced by stress (e.g., arsenite, clotrimazole, thapsigargin), viral infection, or G3BP1 expression	1A	RBPs (e.g., G3BP) and translation factors (e.g., eIF3b)	Integrity can be disrupted by cycloheximide or nsP3 ADP-ribosyhydrolase
	Expression of nsP3 HVD domain in the presence of stress	2A (HVD)	nsP3 HVD domain, RBPs (e.g., G3BP) and translation factors (e.g., eIF3b)	Integrity can be disrupted by cycloheximide
	Expression of hydrolase-deficient nsP3 in the presence of stress	3A	Hydrolase-deficient nsP3, RBPs (e.g., G3BP) and translation factors (e.g., eIF3b)	Integrity can be disrupted by cycloheximide
nsP3 condensates	Expression of WT nsP3 in the presence or absence of stress	1E	WT nsP3 and RBPs (e.g., G3BP)	Insensitive to cycloheximide
	Expression of hydrolase-deficient mutant nsP3 in the absence of stress	S2A	Hydrolase-deficient nsP3 and RBPs (e.g., G3BP)	Insensitive to cycloheximide
	Expression of nsP3 HVD domain in the absence of stress	S1D	nsP3 HVD domain, G3BP1	Insensitive to cycloheximide