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. 2021 Feb 1;118(6):e2022751118. doi: 10.1073/pnas.2022751118

Fig. 3.

Fig. 3.

Alu cDNA is synthesized in the cytoplasm. (A and B) Alu retrotransposition assays in the presence of (A) 3TC or nuclear targeted-3TC (Cpep-3TC), or (B) cytoplasmic-targeted 3TC (PA-4–3TC). n = 3. *P < 0.05 by Mann–Whitney U test. Error bars show SEM. (C) Quantification of reverse transcription of Alu RNA by Alu-specific qPCR in cytoplasmic and nuclear fractions of mouse embryonal carcinoma F9 cells. (D) Quantification of Alu cDNA production in heat-inactivated cytoplasmic fractions. n = 7. (E) Quantification of Alu cDNA production in cytoplasmic fractions isolated from mouse L1 siRNA-treated cells. (F) Quantification of Alu cDNA production in cytoplasmic fractions of WT mouse RPE cells incubated with AZT-triphosphate (AZT-TP) or diethyl-AZT (DE-AZT). n = 3. *P < 0.05; ***P < 0.001 by Mann–Whitney U test. Error bars show SEM.