BLF metabolic disturbances with viral infection.
A, Virus infection models. A neurotropic virus, the pseudorabies virus expressing GFP, PRV152, was injected into rat forelimbs as a brain viral infection model. (n = 9). B, Four days after infection, infected neurons were observed in the hippocampus and cerebral cortex M1 zone. Bars: 100 μm. C, Differential‐metabograms of BLF from control rats (n = 10) and viral infection rats (n = 9). Metabolites with red color are significantly changed between groups. MUDA were used to evaluate the significance of difference in metabolite concentrations between groups. D, In the BLF of the viral infection group, dimethylamine (DMA) and N,N‐dimethylglycine (DMG) were significantly lower, while glutamine was significantly higher than that of the control group. Student's t‐test was used to evaluate the significance of difference in metabolites between control rats and viral infection rats. *P < 0.05.