Table 2.

Pathology antigen retrieval and antibody table. Optimal conditions for the detection of hyperphosphorylated tau, beta amyloid and alpha Synuclein are provided alongside antibodies used for their detection, the corresponding dilution of each antibody and their supplier. Phosphorylated (p) serine (Ser) and Threonine (Thr) residues are provided for AT8 and amino acid (aa) epitope provided for 4G8 and Syn‐1

Pathology co‐stain	Antigen‐retrieval	Pathology antibody	Dilution	Supplier
Hyperphosphorylated Tau	Citrate buffer (10 minutes microwave heated)	AT8 (pSer202/pThr205)	1:4000	Innogenetics, Ghent, Belgium
Beta Amyloid	Citrate buffer + 90% Formic acid (1 hour submersion)	4G8 (aa18‐aa22)	1:15 000	Biolegend, London, UK
Alpha Synuclein	Citrate buffer + EDTA buffer (10 mM EDTA, pH 8, 2 minutes pressured heating)	Syn‐1 (aa91‐99)	1:1000	BD Biosciences, San Jose, CA, USA