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. 2019 Nov 6;30(3):495–514. doi: 10.1111/bpa.12798

Figure 7.

Figure 7

C3a delays the differentiation and maturation of OPCs in vitro by activating the WNT/β‐catenin pathway. C3a administration activates the WNT/β‐catenin pathway in primary culture of OPCs. Panel A shows TCF4, β‐catenin, β‐catenin phosphorylation and AXIN2 immunoreactive bands after C3a treatment for 15 minutes, 30 minutes, 1, 2, and 4 h and the corresponding control (n = 6). Bar graphs (B–E) show the optical density of protein expression in A (n = 6). XAV939, an inhibitor of WNT/β‐catenin pathway, reverses the effect of C3a in primary culture of OPCs. Panel F shows the immunoreactive bands of PLP (26 kDa), MBP (33 kDa), CNPase (47 kDa), MAG (69 kDa), NG2 (251 kDa), CC1 (310 kDa), Olig1 (26 kDa), Olig2 (37 kDa), SOX10 (49 kDa) and β‐actin (42 kDa) after C3a, C3a + C3aRa, C3a + XAV939, XAV939 treatment and the corresponding control (n = 6). Bar graphs (G–O) show the optical density of protein expression in I (n = 6). For statistical analysis, one‐way ANOVA with Tukey's post hoc test was used for B–E and G–O and presented as the mean ± standard error of measurement (SEM). Scale bars: 20 μm. *P < 0.05, **P < 0.01.