. 2021 Feb 13;31(2):365–380. doi: 10.1111/bpa.12927

TABLE 2.

Distribution of distinct populations of tumor cells, TAM (microglial cells and blood‐derived macrophages), and other tumor‐infiltrating immune cells in GBM samples (n = 55)

Cell population and phenotype	Marker	% cells in the tumor sample	% cells within the parental population
Total GBM tumor cells (GFAP⁺)		73% ± 16%
SOX2⁻ GBM tumor cells (CD45⁻ GFAP⁺ SOX2⁻)		52% ± 25%
	EGFR⁺	9% ± 13%	14% ± 26%
	CD24⁺	16% ± 22%	24% ± 32%
	CD44⁺	41% ± 30%	61% ± 34%
	CD192⁺	13% ± 11%	27% ± 26%
SOX2⁺ GBM tumor cells (CD45⁻GFAP⁺SOX2⁺)		20% ± 26%
	CD133⁺	8% ± 10%	48% ± 41%
Immune cells (CD45⁺)		24% ± 18%
Myeloid cells		22% ± 17%	90% ± 15%
TAM (HLA‐DR⁺CD14⁺)		13% ± 12%	61% ± 28%
M1‐like TAM (CD192⁺)		4% ± 6%	17% ± 18%
M2‐like TAM (CD163⁺)		3% ± 6%	16% ± 20%
	CD206⁺	2% ± 3%	12% ± 20%
CD33⁺		13% ± 13%	45% ± 29%
CD16⁺		4% ± 8%	16% ± 20%
CD44⁺		8% ± 8%	35% ± 30%
CD68⁺		9% ± 9%	42% ± 36%
Microglial cells (HLA‐DR⁺CD14⁺ GAFL⁺)		10% ± 11%	44% ± 27%
Blood‐derived macrophages (HLA‐DR⁺CD14⁺ GAFL^lo−)		3% ± 5%	16% ± 20%
Neutrophils (CD15^hi/CD16^hi HLA‐DR⁻)		5% ± 9%	20% ± 21%
MDSC ( CD15^lo CD16⁻ HLA‐DR⁻)		4% ± 8%	15% ± 23%
TIL		2% ± 2%	10% ± 15%
T‐cells (CD3⁺)		1.4% ± 2%	8% ± 9%
CD4⁺CD8⁻ T‐cells		0.5% ± 0.7%	2% ± 4%
CD8⁺CD4⁻ T‐cells		0.6% ± 0.7%	5% ± 7%
CD8⁻CD4⁻ T‐cells		0.2% ± 0.4%	2% ± 4%
CD4⁺CD25⁺CD127⁻ T‐regs		0.1% ± 0.2%	0.4% ± 0.5%
B‐cells (CD19⁺CD20⁺)		0.1% ± 0.2%	0.5% ± 1%
NK‐cells (CD3⁻ CD16⁺CD56⁺)		0.05% ± 0.05%	1% ± 5%

Results expressed as mean ± SD percentage of cells from the whole tumor sample cellularity and from the parental cell population (either tumor cells or all immune cells).

Abbreviations: GAFL, green autofluorescence; TAM, Tumor‐associated macrophages (i.e. microglial cells and blood‐derived macrophages); TIL, Tumor‐infiltrating lymphocytes.