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A. Transversal ON sections stained to detect demyelination (LFB), axonal loss (SMI312) and activated microglia/macrophages (Iba1) in H and EAE rats at 70 dpi (magnification: 10×). Quantification of demyelination (B), axonal loss (C) and Iba1+ cells (D) detected in ONs from H (n = 6) and EAE eyes (n = 12) with recorded VEPs at 70 dpi (***: P < 0.001; ****: P < 0.0001). Data are expressed as mean ± SEM.
