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. 2020 Aug 6;31(1):103–119. doi: 10.1111/bpa.12883

Figure 3.

Figure 3

PKR inhibition decreases soluble tau phosphorylations in transgenic rTg4510 mice brain slices. A. Immunoblot of RIPA‐buffer extracts of acute brain slices using total‐ and pTau‐specific antibodies. Acute brain slices from transgenic rTg4510 mice 2–5 months of age were treated for 4 h with 15 μM PKRi or DMSO as vehicle control. B and C. Quantifications of the effect of PKR inhibition on total tau/TUJ1 (B) or phospho‐tau epitopes/total tau (C) from three independent experiments of brain slices prepared from transgenic rTg4510 mice 2–5 months of age. For each experiment two technical replicates were performed (n = 3 biological replicates, *P < 0.05, student t‐test). D. Immunoblot of acute brain slices from transgenic using total‐ and pTau‐specific antibodies. Acute brain slices from rTg4510 mice of 6‐ (left) or 9 months of age (right) were treated for 4 h with 15 μM PKRi or DMSO as vehicle control. Before immunoblotting brain slices were fractionated into TBS soluble and sarkosyl insoluble fractions.