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. 2020 Aug 6;31(1):103–119. doi: 10.1111/bpa.12883

Figure 5.

Figure 5

Silencing of PKR decreases endogenous tau mRNA expression and protein levels in SH‐SY5Y cells. A. Immunoblot of whole‐cell lysate from SH‐SY5Y cells using PKR and total tau antibodies. Cells were transfected with siPKR RNA or non‐interfering siNon RNA for 48 h. prior to analysis. B. mRNA levels in RNA extracts were quantified by qPCR. PKR mRNA levels are normalized to NADH mRNA. The values in the figure exhibit the ratio between cell transfected with siNon and siPKR (n = 4, **P < 0.01, student t‐test). C. mRNA levels in RNA extracts were quantified by qPCR. Tau mRNA levels are normalized to NADH mRNA. The values in the figure exhibit the ratio between cell transfected with siNon and siPKR (n = 4, *P < 0.05, student t‐test). D. Quantifications of total tau/α‐tubulin from four independent experiments (n = 4, *P < 0.05, student t‐test).