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. 2020 Aug 6;31(1):103–119. doi: 10.1111/bpa.12883

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© 2020 The Authors. Brain Pathology published by John Wiley & Sons Ltd on behalf of International Society of Neuropathology

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PKR reduces the affinity of tau to microtubules. A. Immunoblot of HEK293T cells using total‐ and pTau‐specific antibodies as well as anti‐V5 tag to detect V5‐tagged constitutively active D328A PKR. Cells were transfected with 2N4R tau and TD tomato or 2N4R tau and V5‐tagged constitutively active D328A PKR for 24 h. The immunoblot was performed on fractionated samples (cytosol and microtubule fractions). B. Quantification of percentage of total tau observed in the microtubule fraction (n = 4, *P < 0.05, student t‐test).