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. 2021 Apr 1;106(4):1188–1192. doi: 10.3324/haematol.2020.259275

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Copyright© 2021 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Figure 2. — P66T UBE2T is a partial loss-of-function variant. (A) Immunoblot with anti-HA antibody of RA2627 (UBE2T^-/-) primary fibroblasts expressing empty vector (EV) or C-HA-FLAG P66T UBE2T or wild-type (WT) UBE2T. (B) Cell survival of RA2627 (UBE2T^-/-) fibroblasts expressing EV, P66T UBE2T, or WT UBE2T after treatment with mitomycin C (MMC). (C) FANCD2 ubiquitination with and without MMC treatment in RA2627 (UBE2T^-/-) fibroblasts expressing EV, P66T UBE2T, or W T UBE2T. (D) Quantification of FANCD2 foci formation af ter MMC treatment in RA2627 (UBE2T^-/-) fibroblasts expressing EV, P66T UBE2T, or W T UBE2T. Approximately 300 HA-expressing cells were analyzed for the presence of FANCD2 foci in three separate coverslips. The mean percent nuclei with FANCD2 foci was plotted and tested for signif icance using one-way analysis of variance with multiple comparisons. ns: not significant, ****P=≤0.0001.