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. 2020 Mar 5;106(4):1056–1066. doi: 10.3324/haematol.2019.241026

Figure 4.

Figure 4.

Lymphocyte cell-specific kinase (LCK) knockdown leads to cell cycle arrest in T-cell acute lymphoblastic leukemia (T-ALL) cell lines and patient-derived xenograft (PDX) cells. (A-C) Cell cycle status was determined by flow cytometry using Hoechst 33342 in cell lines Jurkat (A and C), MOLT4 (B and C), and SUPT1 (C) 7 days after transduction with shLCK#3 or shNTC expression vectors. (D) PDX L963 cells were lentivirally transduced with shLCK#3 or shNTC expression constructs. Phosflow analysis of total LCK and p-Y416SRC was performed 8 days later. (E) shLCK#3 and shNTC transduced PDX L963 cells were loaded with CTV and cultured for 13 days. Flow cytometric analysis of CTV incorporation (cell divisions) was performed and demonstrated progressive reduction in cell number after 4-6 cell divisions after LCK knockdown relative to control knockdown. Student's t-test: *P<0.05, **P<0.01, ***P<0.005.