Table1: Fluorescence parameters for IDP/polyproline samples.
Sample | δ | τD | α | τA | rss(donor) | rss(acceptor) | 〈E〉 | 〈S〉 | |
---|---|---|---|---|---|---|---|---|---|
Free-Dyes | 0.035 | 3.92 | 0.041 | 3.89 | * | * | - | - | |
Reference Data Set | P40 | 0.045 | 3.998 | 0.053 | 3.939 | 0.019 | 0.016 | 0.175 | 0.504 |
P27 | 0.040 | 3.994 | 0.043 | 3.851 | 0.006 | 0.015 | 0.336 | 0.503 | |
P20 | 0.040 | 3.948 | 0.048 | 3.937 | 0.011 | 0.011 | 0.563 | 0.502 | |
P14 | 0.050 | 3.794 | 0.020 | 3.899 | 0.022 | 0.007 | 0.774 | 0.502 | |
P11 | 0.038 | 3.983 | 0.047 | 3.898 | 0.021 | 0.020 | 0.863 | 0.497 | |
P6 | 0.045 | 3.951 | 0.020 | 3.767 | 0.020 | 0.016 | 0.952 | 0.506 | |
sNh− | 0.043 | 3.984 | 0.053 | 4.288 | 0.045 | 0.026 | 0.529 | 0.501 | |
sNh+ | 0.040 | 3.966 | 0.051 | 4.001 | 0.038 | 0.014 | 0.318 | 0.494 | |
average | 0.043 | 3.95 | 0.042 | 3.94 | 0.023 | 0.016 | - | 0.501 | |
standard deviation | 0.004 | 0.07 | 0.014 | 0.15 | 0.013 | 0.006 | - | 0.004 | |
Integrase Data Set | IN-WDA | 0.042 | 3.37 | 0.055 | 4.11 | 0.049 | 0.021 | 0.654 | 0.435 |
IN-FDA | 0.057 | 3.66 | 0.047 | 4.09 | 0.030 | 0.031 | 0.649 | 0.480 | |
IN-WAD | 0.044 | 3.81 | 0.047 | 4.26 | 0.032 | 0.038 | 0.633 | 0.506 |
defined as zero for the purpose of correcting for differential collection efficiencies of the parallel and perpendicular detection channels
orange values in the Integrase Data Set are more than 3 standard deviations away from the mean of the Reference Data Set.