Figure 5.

Transfection of shHMGB1 knocked down the levels of HMGB1 in human endometrial stromal cells (HESCs) from endometriosis patients. (A–C) The HESCs were isolated from the ectopic endometrium. Next, the cells were transfected with shNC or shHMGB1 for 24 h. The mRNA and protein levels of HMGB1 were determined using qRT-PCR (n = 4) and Western blotting, separately, in the HESCs. The experiments were repeated independently for three times. The expressions were normalized to GADPH. The data were expressed as means ± SD. One-way ANOVA followed Dunnett’s T3 multiple comparisons test was performed. *P < 0.05 indicates significant difference. ns indicates no significant difference.