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. 2021 Mar 19;12:616696. doi: 10.3389/fendo.2021.616696

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Copyright © 2021 Huang, Chen and Lv

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The knockdown of HMGB1 suppressed inflammatory cytokines in the HESCs under hypoxic conditions. (A–C) The HESCs were transfected with shNC or shHMGB1 for 24 h followed by incubating under hypoxic conditions for another 24 h. The secreted and cellular HMGB1 were determined using ELISA and Western blotting, separately (n = 12). The expressions were normalized to GAPDH. (D–F) The levels of inflammatory cytokines (IL-6, TNF-α, and IL-1β) were determined using specific ELISAs (n = 12). The HESCs that were transfected with shNC were cultured in normoxia. The data were expressed as means ± SD. One-way ANOVA followed Dunnett’s T3 multiple comparisons test was performed. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant difference.