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. 2021 Mar 19;12:616696. doi: 10.3389/fendo.2021.616696

Figure 7.

Figure 7

The knockdown of HMGB1 regulated autophagy in the HESCs under hypoxic conditions. (A) The HESCs were transfected with shNC or shHMGB1 for 24 h followed by incubating under hypoxic conditions for another 24 h. The Representative illustrations of Western blotting showed the protein expressions of Beclin 1, Atg 13, LC3-I, LC3-II, and p62. (B–E) The expression levels of Beclin 1, Atg 13, LC3-I, LC3-II, and p62 in the shHMGB1-transfected HESCs under hypoxia conditions (n = 4). The HESCs that were transfected with shNC were cultured in normoxia. The expressions were normalized to GAPDH. The data were expressed as means ± SD. One-way ANOVA followed Dunnett’s T3 multiple comparisons test was performed. *P < 0.05 and **P < 0.01 indicate significant difference.