Figure 4.

miR-22 directly binds to lncRNA NBR2 and TCF7 (a) A schematic diagram showing the process of identifying miRNAs related to lncRNA NBR2 and TCF7. (b) The expression of miR-22 in normal liver tissues (n = 8) and hepatoblastoma tissues (n = 53) according to GSE75283. (c) The expression of miR-22 was determined in 15 pairs of adjacent non-cancerous tissues and hepatoblastoma tissues by real-time qPCR. (d) The correlation of miR-22 with lncRNA NBR2 and TCF7 expression, respectively, in tissue samples was analyzed using Pearson’s correlation analysis. (e) The expression of miR-22 was determined in L02, HepG2, and Huh-6 cells using real-time qPCR. (f) HepG2 cells were transfected with si-NBR2 and examined for the expression of miR-22 using real-time qPCR. (g) HepG2 cells were transfected with miR-22 mimics or miR-22 inhibitor to achieve miR-22 overexpression or inhibition. The transfection efficiency was determined by real-time qPCR. (h) HepG2 cells were transfected with miR-22 mimics or miR-22 inhibitor and examined for the protein levels of TCF7 by Immunoblotting. (i) The reporter plasmids were transfected in 293 T cells with miR-22 mimics or miR-22 inhibitor and examined for the luciferase activity. **P < 0.01, ##P < 0.01