Figure 5.

Dynamic effects of lncRNA NBR2 and miR-22 on hepatoblastoma cell phenotype HepG2 cells were co-transfected with si-NBR2 and miR-22 inhibitor and examined for (a) the protein levels of TCF7 by Immunoblotting; (b) cell viability by MTT assay; (c) cell invasion by Transwell assay; (d) cell migration by wound healing assay. **P < 0.01, compared to the si-NC + inhibitor-NC group; ##P < 0.01, compared to the si-NBR2 + miR-22 inhibitor group. (e) HepG2 cells were cultured in a medium containing 0 mM or 25 mM glucose and examined for the expression of miR-22 and TCF7 mRNA by real-time qPCR. **P < 0.01. (f) HepG2 cells were co-transfected with si-NBR2 and miR-22 inhibitor, cultured in a medium containing 0 mM glucose, and examined for cell apoptosis by Flow cytometry assay. **P < 0.01, compared to the si-NC + inhibitor-NC group; ##P < 0.01, compared to the si-NBR2 + miR-22 inhibitor group