Figure 3.

Neurological scores, TEER, Ca²⁺ concentrations, and cell permeability in MCAO injury rat model. (a) Neurological scores of the rats in each group. ***P < 0.001 vs MCAO; $$P < 0.01 and $$$P < 0.001 vs curcumin+AS2521780. (b) Ca²⁺ concentration, (c) TEER, and (d) cell permeability were analyzed. #P < 0.05, ##P < 0.01, and ###P < 0.001 vs control; **P < 0.01 and ***P < 0.001 vs MCAO; $P < 0.05, $$P < 0.01, and $$$P < 0.001 vs curcumin+AS2521780. (e) Western blot assay was performed to measure Orai1, PKC-θ, p- PKC-θ, ZO-1, occludin, IκBα, p-IκBα cleaved caspase 3, and nuclear p65 levels. *P < 0.05, **P < 0.01, and ***P < 0.001 vs MCAO; $P < 0.05, $$P < 0.01, and $$$P < 0.001 vs curcumin+AS2521780