Table 1.
Primer Sequences Specifications Used in This Study
| Primer | Sequences | T* (°C) | |
|---|---|---|---|
| Caspase-9 | Forward | 5ʹ-AAGTGACCCTCCCAAGTAGC-3’ | 60.5 |
| Reverse | 5ʹ-GTTCTGGCCAGGTCTCTTCT-3’ | ||
| Caspase-3 | Forward | 5ʹ-AAAATACCAGTGGAGGCCGA-3’ | 58.4 |
| Reverse | 5ʹ-GCACAAAGCGACTGGATGAA-3’ | ||
| PARP-1 | Forward | 5ʹ-TGGAACATCAAGGACGAGCT-3’ | 60.5 |
| Reverse | 5ʹ-CATCGCTCTTGAAGACCAGC-3’ | ||
| BCL-2 | Forward | 5ʹ-TCCTCTTTACACTGGCCAGG-3’ | 60.5 |
| Reverse | 5ʹ-GAGTATTTGTGCAGCGAGGG-3’ | ||
| GAPDH | Forward | 5ʹ-AAGGTGAAGGTCGGAGTCAAC-3’ | 57.3 |
| Reverse | 5ʹ-CTTGATTTTGGAGGGATCTCG-3’ | ||
Note: *T = annealing temperature.