Figure 4.

In vitro effects of p65 on trophoblast inflammatory factors (a-b) P65 silencing was achieved in HTR-8/SVneo and JEG-3 cells by transfecting small hairpin RNA for p65 (sh-p65); the transfection efficiency was verified by real-time qPCR and Immunoblotting. Then, HTR-8/SVneo and JEG-3 cells were transfected by sh-p65, exposed to LPS, and examined for the mRNA levels of TNF-α and IL-6 by real-time qPCR (c). *P< 0.05, **P< 0.01, compared with the control group; ##P< 0.01, compared with the sh-NC + LPS group