Figure 2.

Dynamic effects of p53 and miR-129 on p53, p21, and RB cells. (a) miR-129 expression in two RB cell lines achieved by transfection of miR-129 mimics or miR-129 inhibitor, as confirmed by qPCR. Two RB cell lines were co-transfected with si-p53 and miR-129 mimics, the protein levels of p53, p21, Bax, Bcl-2, and cleaved-caspase 3 were examined by Immunoblotting (b–d); the cell viability of co-transfected cells examined by MTT assays (e–f); the DNA synthesis capacity of co-transfected cells examined by FACS analysis (g–h); the cell apoptosis was examined by Flow cytometry (i–j). The data are presented as mean ± SD of three independent experiments. *P< 0.05, **P< 0.01, compared to control group; #P< 0.05, ##P< 0.01, compared to miR-129 mimics + si-NC (negative control) group