Figure 3.

miR-129 directly targets MDM2/4 and affects p53 signaling (a–d) Y79 and WERI-RB-1 cells were transfected with miR-129 mimics or miR-129 inhibitor and examined for the protein levels of MDM2 and MDM4. (e–g) Wild-type or mutant-type MDM2/4 3’-UTR vector containing wild or mutated miR-129 binding site was constructed and co-transfected into Y79 and WERI-RB-1 cells, respectively, with miR-129 mimics or miR-129 inhibitor. The luciferase activity was examined. (h–k) MDM2/4 overexpression in Y79 and WERI-RB-1 cells was achieved by transfection of MDM2/4 overexpressing vector, as confirmed by Immunoblotting. Y79 and WERI-RB-1 cells were co-transfected with miR-129 mimics and MDM2/4 overexpressing vector and examined for the protein levels of MDM2/4, p53, p21, Bax, Bcl-2, and cleaved-caspase 3. The data are presented as mean ± SD of three independent experiments. *P< 0.05, **P< 0.01, compared to control group; #P< 0.05, ##P< 0.01, compared to miR-129 mimics + NC group