Figure 3.

Constructing heterogeneous structures. (A) Structure with multiple agarose concentrations. (Ai) Photo of agarose structure (dyed for visualization, with each color representing a different concentration) in ascending layers. Layers are 500 μm thick, and each color contains 5 layers. (Aii) Cross-sectional schematic showing the agarose concentrations in each layer. (B) Purple agarose heart inside a colorless agarose cube demonstrating the ability to pattern materials of different composition within a layer (left). Schematic of patterning layer 15 of the 34-layered structure with compartmentalization features to segregate the different gels (right). Layers are 500 μm thick.