Skip to main content
. Author manuscript; available in PMC: 2021 Apr 2.
Published in final edited form as: J Parkinsons Dis. 2020;10(4):1411–1427. doi: 10.3233/JPD-202241

Fig 1. Experimental design for α-Syn seeding and subsequent multi-site LFP recordings in awake mice.

Fig 1.

A, 2–3 months old C57BL/6J mice received unilateral OB injections of either α-Syn PFFs or PBS and survived for 1, 2, or 3 months post injection. The mice were then surgically implanted ipsilaterally to the initial surgical site in the OB and PCX with twisted bipolar electrodes for LFP recordings. Following this, the mice were allowed 2–3 days to recover. Spontaneous and odor-evoked LFPs were recorded from awake mice while they were head fixed (to allow control for the positioning of the snout relative to the odor port) in the absence and presence of odors respectively, and then were perfused within 3 days for post-mortem histology. Image made in BioRender. B, Example localization of the electrode implants in the OB and PCX of a mouse injected with PBS using 10X magnification. * = former sites of bipolar electrode tips.