Figure 4.

AP firing numbers in small-sized V2 TG neurons of sham group and ION-CCI group. A, Two sets of sample traces show two types of AP firing patterns recorded from small-sized DiI-labeled V2 TG neurons, the single AP firing pattern (left) and the multiple AP firing pattern (right). The two sets of traces were from two small-sized DiI-labeled V2 TG neurons of the sham group. B, Percentages of small-sized DiI-labeled V2 TG neurons showing single AP firing pattern (open bars) and multiple AP firing pattern (closed bars) in the sham group (n = 24) and the ION-CCI group (n = 26). C, Numbers of APs in small-sized DiI-labeled V2 TG neurons with multiple AP firing in the sham (n = 24, closed circles) and the ION-CCI groups (n = 26, closed squares). In A-C, APs were elicited by stepwise currents of 2 times of AP rheobases. D, Sample traces show APs evoked by ramp currents injected via recording electrodes in a small-sized DiI-labeled V2 TG neuron of the sham group (left) and the small-sized DiI-labeled V2 TG neuron of ION-CCI group (right). Inset, the ramp current amplitude and duration. E, Percentages of small-sized V2 TG neurons showing single AP firing pattern (open bars) and multiple AP firing pattern (closed bars) in the sham group (n = 20) and the ION-CCI group (n = 16). F, Numbers of APs of small-sized DiI-labeled V2 TG neurons with multiple AP firing in the sham group (n = 20, closed circles) and the ION-CCI group (n = 16, closed squares). In E, F, APs were elicited by ramp currents. Data represent mean ± SEM; NS, no significant difference; **p < 0.01.