Figure 8.
Gemfibrozil protects striatal catecholamine levels and restores behavioral function in MPTP-lesioned Gfapcre mice, but not Gdnf Δastro mice. The 6- to 8-week-old Gfapcre mice or Gdnf Δastro mice (n = 6/group) were lesioned with MPTP (20 mg/kg body weight/injection, four intraperitoneal injections at every 2 h interval). A–C, At 4 h following the last MPTP injection, mice were fed gemfibrozil (7.5 mg/kg body weight/d) via gavage for 7 d. Analysis of dopamine (DA), DOPAC, and HVA in striatum. Results are the mean ± SD of 6 mice/group. A two-way ANOVA found F(2,30) = 82.08 > Fc = 3.13 (****p < 0.0001) for genotype and F(1,30) = 18.12 > Fc = 4.17 (***p = 0.0002) for treatment. D, Gfapcre mice, but not Gdnf Δastro mice, displayed significant improvement with respect to motor coordination, as tested on Rotarod. A two-way ANOVA results in F(2,30) = 80.40 > Fc = 3.13 (****p < 0.0001) for treatment and F(1,30) = 13.38 > Fc = 3.13 (****p < 0.0001) for treatment. E, Open field analysis. F, Supported rearing. A two-way ANOVA results in F(2,30) = 77.89 > Fc = 3.13 (****p < 0.0001) for treatment and F(1,30) = 16.84 > Fc = 3.13 (****p < 0.0001) for treatment. G, Grooming counts observed in the 5 min open field test. A two-way ANOVA results in F(2,30) = 31.04 > Fc = 3.13 (****p < 0.0001) for treatment and F(1,30) = 10.29 > Fc = 3.13 (**p = 0.0032) for treatment. Post hoc Sidak's multiple-comparisons tests were used to identify significant differences among different groups (****p < 0.0001).