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Primer sequences of the genes.
| Genes | Primer sequences | Sequence length (bp) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| BDNF F | GATCCACTGAGCAAAGCCGA | 20 | 137 | 59.8 |
| BDNF R | CTCACCTGGTGGAACATTGTG | 21 | ||
|
| ||||
| c-fos F | CCAAGCGGAGACAGATCAAC | 20 | 138 | 58.8 |
| c-fos R | CCCAGGTCATTGGGGATCTT | 20 | ||
|
| ||||
| β-Actin F | ACGGTCAGGTCATCACTATC | 20 | 90 | 56.5 |
| β-Actin R | TGCCACAGGATTCCATACC | 19 | ||
