Figure 2.

FGFRs are required early in the neurogenic cell lineage of the DG. A–R, Experimental timelines: A for B, C, O; D for E–H, N, P); and I for J–M, Q, R). B–M, Immunostains of the DG of mice in home cages, for control and Fgfr-3KO at 5 d (B, C), 2 weeks (E–H), 3 weeks (J, K), and 5 weeks (L, M) post-tamoxifen injection. In G, H: BrdU, white; DCX, red; DAPI, blue; GFAP, green; in J–M: NeuN, green. Scale bar, 50 µm. N–R, Number of BrdU⁺DCX^– RGL/early progenitor cells 2 weeks post-tamoxifen injection (N), BrdU⁺DCX⁺ immature neurons/late progenitors 5 d post-tamoxifen injection (O), BrdU⁺DCX⁺ immature neurons/late progenitors 2 weeks post-tamoxifen injection (P), BrdU⁺NeuN⁺ mature neurons 3 weeks post-tamoxifen injection (Q), and BrdU⁺NeuN⁺ mature neurons 5 weeks post-tamoxifen injection (R). Two-tailed unpaired Student's t test: *p = 0.022 in N; **p = 0.006 in P; *p = 0.0303 in Q; ****p < 0.0001 in R. Each circle represents a mouse (total number of mice is indicated at the bottom of each bar). Values are the mean ± SEM. wks, Weeks.