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. 2021 Mar 31;41(13):2870–2882. doi: 10.1523/JNEUROSCI.3040-20.2021

Figure 9.

Figure 9.

IL-4-induced actions do not involve IL-10. A, IL-4 (200 ng)-induced antinociception is not reversed by anti-IL-10 (F(3,32) = 1.413, p = 0.2569; one-way ANOVA). N = 9 mice per group. IL-4 was co-injected with anti-IL-10 or control IgG at the CCI site and mechanical von Frey thresholds were measured 5 min after injections, on day 14 after CCI. B, IL-4 (10–200 ng/ml) does not induce IL-10 release from M1 macrophages from injured nerves (all values represent 0). F4/80+ macrophages were isolated by IMS from injured nerves on day 14 after CCI, ex vivo stimulated (5 min) with IL-4 or vehicle (PBS), and extracellular levels of IL-10 were measured by ELISA. N = 8 samples per group. All data are presented as individual data points and mean ± SEM.