Figure 1.

Analysis of Vglut expression in OHCs. A, The Cre-dependent tdTomato (red) fluorescent reporter mouse (Ai14) crossed to Vglut1^IRESCre, Vglut2^Cre, Vglut2^IRESCre, or Vglut3^IRESCre mice indicates expression of VGluT3 in OHCs of the cochlea at P8 and P35, and VGluT2 expression in occasional OHCs in the Vglut2^Cre, but not the Vglut2^IRESCre mouse lines. Hair cells are labeled with myosin VI (green). Scale bar, 20 µm. B, ISH for Vgluts1-3 in OHC, IHC, and SGN in sections of P8.5 mice. Hair cells are labeled with myosin VI (green). Scale bar, 20 µm. C, Cochlea of P8 Prestin^CreERT2;tdTomato mouse injected with tamoxifen shows expression of the fluorescent reporter in OHCs of the cochlear apex, mid-turn, and base. Scale bar, 200 µm.