Figure 2.

BRC-1 and BRD-1 are required for axon regeneration of D-type motor neurons. A, Representative D-type motor neurons in wild-type and brc-1(tm1145) brd-1(dw1) mutant animals 24 h after laser surgery. In wild-type animals, severed axons exhibited regenerated growth cones (yellow arrowheads). In brc-1(tm1145) brd-1(dw1) mutants, the proximal ends of axons failed to regenerate (white arrowheads). Scale bar: 10 µm. B, Percentages of axons that initiated regeneration 24 h after laser surgery at the young adult stage. The number of axons examined is shown. Error bars indicate 95% confidence intervals; **p < 0.01, ***p < 0.001, as determined by Fisher's exact test. NS, not significant. C, Length of regenerating axons 24 h after laser surgery. Data are presented as a box-plot representing median (thick line within the box) and interquartile range (edge of box) with individual data points. The number (n) of axons examined is shown. Statistical significance was determined by Wilcoxon rank-sum test.