Figure 6.

BRC-1–BRD-1 downregulates DGK-3 to promote axon regeneration. A, Isolation of dgk-3 mutants. Genomic structure of the dgk-3 gene is shown. The dgk-3(km89) mutation is a 20-bp insertion (red nucleotides) that contains an in-frame premature stop codon (*), thus terminating translation in the middle of exon 1. The dgk-3(km90) mutation is a 5-bp deletion, causing a frameshift (bold amino acids) and premature stop codon (*) in exon 1. B, Percentages of axons that initiated regeneration 24 h after laser surgery at the young adult stage. The number of axons examined is shown. Error bars indicate 95% confidence intervals; *p < 0.05, **p < 0.01, as determined by Fisher's exact test. NS, not significant.