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. 2020 Jul 28;34(2):249–260. doi: 10.1007/s10334-020-00868-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Exemplary image detailing the region-of-interest (ROI) definition. Water saturation shift referencing sequence (WASSR) image of the tibiotalar joint of a 29-year-old healthy male. Manual definition of the ROI in the central weight-bearing region of the tibiotalar joint was performed individually by two radiologists to include the cartilage layers of the tibiotalar joints while reducing partial volume artifacts due to cortical bone and/or joint fluid