Table 1.
Sequences of primers used for evaluation of SHH signaling genes and their regulatory microRNAs.
| Accession Number | Gene Name | Primers 5′ → 3′ |
|---|---|---|
| NM_002046.7 | GAPDH | Specific forward primer: |
| TGGAGTCCACTGGCGTCTTCAC | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACAGGCATTGCTGA | ||
| NM_002181.4 | IHH | Specific forward primer: |
| CCCGTCGTGGTGTAGTCATAGAG | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACAGGCTGAGTTGG | ||
| NM_001301861.2 | BOC | Specific forward primer: |
| CACTGGCTTGCCTCCTCCTA | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACGCATCATCCGAG | ||
| NM_016277.5 | RAB23 | Specific forward primer |
| GCTTGTGTGCTCGTGTTCTCTAC | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACACTTCGGCTACTAC | ||
| NR_106796.1 | MIR6738 | Specific forward primer |
| CGACCTTCTGCCTGCATTCTA | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACCTGGGAG | ||
| NR_029712.1 | MIR195 | Specific forward primer |
| GCGGCTAGCAGCACAGAAA | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACGCCAATA | ||
| NR_030629.1 | MIR509-3 | Specific forward primer |
| CGCATACTGCAGACGTGG | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACCATGATTG | ||
| NR_004394.1 | RNU6-1 | Specific forward primer |
| CTCGCTTCGGCAGCACATATAC | ||
| RT-PCR primer | ||
| GTCGTATCCAGTGCTGCGACCGTATGGATGTGTCTGCGGCGTTTTATCATGCACTGGATACGACGTGTCATCCTTGC |
The target specific portion of mRNA and micro-RNA is showed in red bold. Stem-loop sequence is underlined. Tm of the micro-RNA forward primer was increased by adding a tail (green sequences) to the 5′-end of the sequences.