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. 2020 Aug 9;12(4):261–278. doi: 10.1007/s13238-020-00751-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HDAC3 positively regulates type I interferon production. (A) Luciferase activity in shNC, shHDAC3-1 or shHDAC3-2 cell lines (1 × 10⁵), transfected for 36 h with a luciferase reporter for IFN-β (IFN-β-luc) or for ISRE (ISRE-luc), uninfected or infected with SeV for 10 h before luciferase assays were performed. HDAC3-Knockdown efficiency in the shHDAC3 cell lines were confirmed by immunoblotting analysis (lower). (B) ELISA analysis of IFN-β in the supernatant of shNC, shHDAC3-1 cell lines, followed by infection with or without SeV, VSV, HSV-1. (C) ELISA of IFN-β in the supernatant of THP-1 HDAC3-deficient cells (HDAC3 KO-1, HDAC3 KO-2), followed by infection with different stimulus (as shown). HDAC3 knockout efficiency in the THP-1 cells were confirmed by immunoblotting analysis (right). (D) Luciferase activity assay in HEK293T cells (1 × 10⁵), co-transfected for 36 h with empty vectors, increased amounts of HDAC3 expression plasmids or Flag tagged HDAC3-H134Q (100 ng) together with a luciferase reporter for IFN-β (IFN-β-luc) or for ISRE (ISRE-luc), followed by infection with or without SeV for 10 h before luciferase assays were performed. (E) ELISA analysis of IFN-β in the supernatant of HEK293T cells (1 × 10⁵), transfected for 36 h with empty vectors, increased amounts of HDAC3 expression plasmids or Flag tagged HDAC3-H134Q (100 ng), followed by infection with or without SeV for 10 h. (F) ELISA of IFN-β analysis in the supernatant of HDAC3-stable-expressing THP-1 cells, followed by infection with different stimulus (as shown). Data are representative of three independent experiments. Graphs show mean ± SD; n = 3. *P < 0.05; ** P < 0.01; ***P < 0.001 (Student’s t-test)