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. 2020 Jul 14;12(4):279–296. doi: 10.1007/s13238-020-00753-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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POST1 promotes self-cleavage of S1P at the C’/C sites. (A) Venn diagram showing POST1-interacting proteins from three independent co-immunoprecipitation experiments coupled to tandem mass spectrometry. Top 15 commonly detected protein hits were listed in details. (B) HeLa cells were transfected with pCMV-S1P-5×Myc and increasing amounts of pCMV-POST1-3×Flag as indicated for 48 h and subjected to the co-immunoprecipitation assay with the anti-Flag agarose. (C) Schematic representation of the S1P precursor with a C-terminal Flag tag. Amino acid numbers of human S1P are shown. Signal peptidase cleavage site (A), S1P autocatalytic cleavage sites (B’/B and C’/C) and enzymatic sites are indicated. SP, signal peptide; TM, transmembrane domain; BM, B’/B cleavage site mutations; CM, C’/C cleavage site mutations; EM, enzymatic site mutations. (D–F) HeLa cells were transfected with the indicated plasmids for 48 h and subjected to immunoblotting

POST1 promotes self-cleavage of S1P at the C’/C sites. (A) Venn diagram showing POST1-interacting proteins from three independent co-immunoprecipitation experiments coupled to tandem mass spectrometry. Top 15 commonly detected protein hits were listed in details. (B) HeLa cells were transfected with pCMV-S1P-5×Myc and increasing amounts of pCMV-POST1-3×Flag as indicated for 48 h and subjected to the co-immunoprecipitation assay with the anti-Flag agarose. (C) Schematic representation of the S1P precursor with a C-terminal Flag tag. Amino acid numbers of human S1P are shown. Signal peptidase cleavage site (A), S1P autocatalytic cleavage sites (B’/B and C’/C) and enzymatic sites are indicated. SP, signal peptide; TM, transmembrane domain; BM, B’/B cleavage site mutations; CM, C’/C cleavage site mutations; EM, enzymatic site mutations. (D–F) HeLa cells were transfected with the indicated plasmids for 48 h and subjected to immunoblotting