Skip to main content
. 2021 Apr 3;10(4):e1256. doi: 10.1002/cti2.1256

Figure 1.

Figure 1

Reconstitution of patient serum with purified factor D increases complement C3 binding to the bacterial surface and complement‐mediated killing. (a) N. meningitidis serogroup B strain H44/76 (MenB) (n = 6), N. meningitidis serogroup C strain C11 (MenC) (n = 6), S. pneumoniae strain PBCN0460 (Sp 0460) (n = 4), S. pneumoniae strain TIGR4 (Sp TIGR4) (n = 4), NTHi strain 11P6H (n = 4) and NTHi strain 86‐028NP (n = 4) were incubated with 10% patient serum in HBSS3+ with and without factor D reconstitution for 30 min at 37°C, and binding of C3 to the bacterial surface was determined by flow cytometry. (b) N. meningitidis serogroup B strain H44/76 (MenB) (n = 6), N. meningitidis serogroup C strain C11 (MenC) (n = 6), NTHi strain 11P6H (n = 4) and NTHi strain 86‐028NP (n = 4) were incubated with 20% (N. meningitidis) or 5% (NTHi) patient serum with and without factor D reconstitution for 60 min at 37°C, and the number of bacteria was determined by colony‐forming unit (CFU) counts. A two‐tailed paired t‐test was performed to determine statistical significance. NS, not significant; *P‐value < 0.05; **P‐value < 0.01.