FIGURE 4.

Increased ductular reaction induced by the HFD may be due to bile duct epithelial cells. A, CDH1^F/F mice crossed with K19CreERT mice, in which a TAM‐inducible CreERT was inserted into the endogenous K19 locus (CDH1^F/F/K19CreERT;CDH1^∆ich). B, At 12 wk after TAM injection, mice were fed with a ND or HFD. H&E and immunostaining results of F4/80, Sox9, and CK19 are shown (×100 magnification). C, Quantification of Sox9‐ and CK19‐positive cells of CDH1^F/F and CDH1^∆ich mice fed the ND or HFD are shown (n = 5 for all groups). D, Rosa26^tdTomato mice were crossed with CDH1^∆ich mice; most of the ductular cells were tdTomato‐positive, suggesting that increased ductular cell expression in HFD‐administered mice was due to BECs (×100 magnification)