FIGURE 3.
Anti‐tumor effects of the anti‐c‐met CAR‐T cells in orthotopic model of human PRCC. A498‐Luc (8 × 105 cells) with Matrigel were injected into the subcapsular space of left kidney of immunodeficient NSG mice on day 0, followed by iv injection of 1 × 106 or 3 × 106 CAR‐T or UTD‐T cells on day 17, or left nontreated (NT). Tumor growth was assessed using IVIS twice a week. A, Representative bioluminescence images of the mice are shown. B, Total flux of whole‐body bioluminescence measured using IVIS is shown as mean ± SEM. Data from 2 independent experiments are combined (NT, UTD‐T, CAR‐T 3 × 106: N = 10 per each group, CAR‐T 1 × 106: N = 5). * and ** represent P values <.05 and <.01, respectively, calculated by two‐sided t test