FIGURE 2.

Inhibition of VAP‐1 enhanced the production of IFN‐γ by CD8⁺ T cells and cytolytic genes in the TME. A‐C, Enhanced production of IFN‐γ in response to EL‐4 cells and the MC‐38 tumor antigen gp70 was observed in CD8⁺ T cells isolated from the tumor tissues, spleens, and draining lymph nodes. The cells were pooled from 7 mice, cultured for a short period with the gp70 peptide, and subjected to ELISA in replicates. Representative data from 4 experiments with similar results are shown. D, Microarray analysis revealed that the inhibition of VAP‐1 upregulated the expressions of cytolytic genes (Prf1, Gzma, Gzmb, and Gzmc) in TME. E, Inhibition of VAP‐1 enhanced cytotoxic activity of tumor antigen‐specific T cells. **P < .01; ***P < .001