FIGURE 3.

ASAP2 promotes cell cycle progression. A, Direct sequencing analysis confirmed successful genome editing of ASAP2 exon 1. Approximately 200 nucleotides, including the initiator codon, were deleted (left). RT‐PCR of gene‐targeted ASAP2. WT: 1220 bp, KO: approximately 1000 bp (top right). ASAP2 western blot analysis in ASAP2 knockout cells and wild‐type cells (bottom right). B, Knockout of ASAP2 suspends cell cycle progression of PDAC cells. Cell cycle assay after refeeding of FBS using flow cytometry in wild‐type cells and ASAP2 knockout cells. n.s. not significant; (*) P < .05; (**) P < .01; (***) P < .001